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R&D Systems Hematology mcd86 fc
Mcd86 Fc, supplied by R&D Systems Hematology, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mcd86+fc/Recombinant+Mouse+B7-2%2FCD86+Fc+Chimera+Protein%2C+CF/bio_rxiv__2023__05__26__542386-237-38-43
Average 91 stars, based on 3 article reviews
mcd86 fc - by Bioz Stars, 2026-10
91/100 stars

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Immunoprecipitation:

Article Title: Soluble CTLA-4 mainly produced by Treg cells inhibits and resolves type 1 inflammation but allows type 2 immunity
Article Snippet: Stained samples were observed with the FluoView FV10i confocal microscope (Olympus Life Science). .. Immunoprecipitation (IP) was performed in binding buffer (50 mM Hepes, pH 7.5, 150 mM NaCl, 5% glycerol, 1% BSA, 0.05% Tween-20) containing 1 μg FLAG-tagged sCTLA-4 and 1 μg mCD80 Fc (human IgG1) chimera (R&D, 740-B1), 1 μg mCD86 Fc (human IgG1) chimera (R&D, 741-B2), or 1 μg Human IgG1 Fc (R&D, 110-HG) in the presence or absence of 1 μg mCD152/Fc (CTLA4-Ig, mouse IgG2a) chimera (Sigma-Aldrich, C4358). .. The anti-human IgG Fc Antibody (BioLegend) and the Sheep Anti-Rat IgG Dynabeads (Thermo Fisher Scientific, 11035) were used for pull-down assay.

Binding Assay:

Article Title: Soluble CTLA-4 mainly produced by Treg cells inhibits and resolves type 1 inflammation but allows type 2 immunity
Article Snippet: Stained samples were observed with the FluoView FV10i confocal microscope (Olympus Life Science). .. Immunoprecipitation (IP) was performed in binding buffer (50 mM Hepes, pH 7.5, 150 mM NaCl, 5% glycerol, 1% BSA, 0.05% Tween-20) containing 1 μg FLAG-tagged sCTLA-4 and 1 μg mCD80 Fc (human IgG1) chimera (R&D, 740-B1), 1 μg mCD86 Fc (human IgG1) chimera (R&D, 741-B2), or 1 μg Human IgG1 Fc (R&D, 110-HG) in the presence or absence of 1 μg mCD152/Fc (CTLA4-Ig, mouse IgG2a) chimera (Sigma-Aldrich, C4358). .. The anti-human IgG Fc Antibody (BioLegend) and the Sheep Anti-Rat IgG Dynabeads (Thermo Fisher Scientific, 11035) were used for pull-down assay.



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R&D Systems Hematology mcd86 fc
Mcd86 Fc, supplied by R&D Systems Hematology, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mcd86+fc/Recombinant+Mouse+B7-2%2FCD86+Fc+Chimera+Protein%2C+CF/bio_rxiv__2023__05__26__542386-237-38-43
Average 91 stars, based on 1 article reviews
mcd86 fc - by Bioz Stars, 2026-10
91/100 stars
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